Figure 3.

mTOR Expression Resulting from Laser-Induced CNV

(A–L) Compared with normal chorioretinal tissue, mTOR expression was upregulated throughout the neural retina and subretinal CNV lesions for both 0.1% PBS-injected control eyes (A–F) and rAAV-scrambled shRNA-injected eyes (G–I), whereas mice treated with mTOR shRNA showed substantially reduced mTOR expression throughout (J–L). (M and N) Quantitative analyses were conducted using RT-PCR. mTOR mRNA levels were substantially increased upon the formation of CNV and subsequently reduced significantly upon the intravitreal administration of rAAV-mTOR shRNA. Data are presented as mean ± SEM. Asterisk indicates statistically significant (*p < 0.05) differences versus the control. Cross indicates statistically significant (^†p < 0.05) differences versus the rAAV-mTOR shRNA-treated group. Scale bar, 100 μm. ONL, outer nuclear layer; RPE, retinal pigment epithelium.