Figure 4.

Immunohistochemistry of Phalloidin and CD31 for Endothelial Cells Resulting from Laser-Induced CNV

The extensiveness of the CNV was measured via whole-mount phalloidin (red) and CD31 (yellow) immunostains. (A–I) Whereas the areas where CNV occurred were widespread for the control and scrambled shRNA-treated groups (A–F), CNV was substantially reduced for mice treated with mTOR shRNA (G–I). Bar graph shows CNV extent, as measured by pixels per lesion, using the Kruskal-Wallis test with post hoc analysis (J, mean ± SEM). Asterisk indicates statistically significant differences versus the control (*p < 0.05). Cross (†) indicates statistically significant differences versus the scrambled shRNA-treated group. Scale bars, 100 μm.