Figure 5.

Transverse Retinal Sections Immunolabeled with CD31 and GFP

(A–L) CD31 (red) expression was detectable throughout the laser-induced CNV lesions in the retinas of the control- (A–D) and rAAV-scrambled shRNA-treated groups (E–H), while CD31 expression was markedly reduced in the rAAV-mTOR shRNA-treated group (I–L). (F and J) Substantial GFP (green) expression exhibited in the rAAV-scrambled shRNA- and rAAV-mTOR shRNA-treated groups (F and J) indicated the effective transducing qualities of the viral vector. Scale bar, 100 μm. ONL, outer nuclear layer; RPE, retinal pigment epithelium.