Figure 6.

Representative Confocal Images of CD11b and F4/80 Immunostaining and Quantitative Analyses of Inflammatory Cell Infiltration

(A–F and J–O) Immunohistochemistry with CD11b and F4/80 revealed the extensive presence of inflammatory cells in the retinas of mice injected with 0.1% PBS and rAAV-scrambled shRNA. (G–I and P–R) In contrast, significantly fewer CD11b- and F4/80-positive inflammatory cells were found in the animals treated with rAAV-mTOR shRNA. (S and T) Significantly fewer CD11b- (S) and F4/80-positive cells (T) were seen in the rAAV-mTOR shRNA-treated group when compared with the control and rAAV-scrambled shRNA-treated groups (CD11b: p = 0.036 and p = 0.016, respectively; F4/80: p = 0.027 and p = 0.022, respectively). Data are presented as mean ± SEM. Asterisk indicates statistically significant differences versus the control (*p < 0.05). Cross (†) indicates statistically significant differences versus the rAAV-scrambled shRNA-treated group. Scale bars, 100 μm. ONL, outer nuclear layer; RPE, retinal pigment epithelium.