Fig. 2.

S100A7 expression was significantly upregulated in breast cancer cells by interaction with adipose stromal cell (ASCs). a Scatterplot analysis of microarray data indicates a total of 435 genes with more than a twofold increase in MCF7 + human adipocyte stromal cells (hASCs) compared with MCF7 cell monoculture are shown above the diagonal line. The table at right shows the top 15 upregulated genes among the group of genes in the scatterplot. b Verification of S100A7 messenger RNA (mRNA) expression by quantitative real-time polymerase chain reaction in MCF7 cells cocultivated with primary human preadipocytes/ASCs compared with monoculture. c Verification of S100A7 mRNA in MCF7 and SK-BR-3 cells cocultured with 3T3-L1 preadipocytes/ASCs compared with monoculture. d Protein expression levels of S100A7 and receptor for advanced glycation endproducts in MCF7, SK-BR-3, T47D, and ZR-75-1 cells cocultivated with 3T3-L1 preadipocytes/ASCs compared with monoculture. ** P < 0.0001 compared with control. The data shown are representative of three independent experiments and shown as mean ± SD