Fig. 5. Binding study examining the formation of pseudorotaxane [L·M] from the individual components L and M (25 mM) in CDCl3 at 0 °C. Partial 1H–1H ROESY NMR (499.9 MHz) correlation spectrum highlights the ROE cross peaks showing through space interactions between the maleimide component and the macrocycle. Unassigned cross peaks represent the through space interactions within the individual components. Cartoon representation shows the association of the macrocycle with the diaryl amide-binding site (red) on the maleimide component.
