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. Author manuscript; available in PMC: 2017 Jun 20.
Published in final edited form as: Cell Rep. 2015 Jun 11;11(11):1694–1702. doi: 10.1016/j.celrep.2015.04.061

Figure 1. CPEB3 Is SUMOylated and Aggregates in Cultured Neurons.

Figure 1

(A) Western blotting (WB) showing detergentinsolubility assay of neuronal cells stimulated for0 and 30min with glycine. Soluble (S) and insoluble(I) fractions were probed with endogenous CPEB3antibody.

(B) Graph showing the percent of CPEB3 in theinsoluble fraction as a mean of five independenttrials ± SE (t- Stud p < 0.0001).

(C) WB showing immunoprecipitation of endogenous CPEB3 from cultured neurons stimulatedwith glycine for 0 and 15 min (CPEB3, immunoprecipitation using an antibody against CPEB3;IgG, IgG negative control; In, Input). The top panelshows WB using an antibody raised againstSUMO-2/3. The bottom panel shows WB using anantibody raised against CPEB3.