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. 2017 Jun 19;14:120. doi: 10.1186/s12974-017-0894-6

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — B1 B cell depletion reduced IgM deposition and complement activation in the injured spinal cord. a Immunofluorescence images of IgM and C3d deposition in cords isolated from control and B1 B cell depleted mice. Coronal step sections (100 um) were obtained across 1 mm from epicenter of contusion injury, and intensity measured and averaged. Composite image (merged) indicates co-localization. Representative images, n = 6. b, c Quantification of IgM binding and C3d deposition, respectively. Mean ± SE, **p < 0.01, n = 6