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. 2016 Aug 19;7:12531. doi: 10.1038/ncomms12531

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a,b) Expression of GluN2A and GluN2B in synaptosome-containing P2 fraction of spinal cord dorsal horn (SDH) of WT and Arrb2-KO mice. (a) Enrichment of synaptic proteins PSD-95 and synaptophysin (SYN) in synaptosome-contacting P2 fraction but not cytosol fraction. (b) Quantification of GluN2A and GluN2B expression. *P<0.05, Student t-test, n=4 mice per group. (c) Surface expression of GluN2B and Arrb2 in HeLa cells transfected with GluN1/GluN2B and GluN1/GluN2B/Arrb2. (d) Relative expression levels of GluN2B and Arrb2, normalized with N-cadherin, a positive control for surface expression. *P<0.05, Student’s t-test, n=3 cultures per group. (e) Pull down assay showing Co-IP of Arrb2 with GluN2B in HeLa cells. All the data are mean±s.e.m. Gel images have been cropped for presentation. Full size images are presented in Supplementary Fig. 8a.