Figure 3.

siRNA-mediated knockdown of TMEM65 in healthy human dermal fibroblast affected mitochondrial function. (a) Oxygen consumption rate was estimated in fibroblasts transfected with control scrambled siRNA (black bars) and TMEM65 siRNA (gray bars). The fibroblasts knocked down for TMEM65 showed significantly lower cellular respiration at baseline, after oligomycin exposure and after FCCP injection when compared with control fibroblasts transfected with scrambled siRNA. Graph represents three individual experiments with different fibroblast cells from three healthy individuals. (b) Patient and TMEM65 siRNA knocked down fibroblasts showed significantly reduced mitochondrial DNA copy number and reduced enzyme activity as shown in representative graph. ***P<0.0001.