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. 2017 Mar 15;25(6):744–751. doi: 10.1038/ejhg.2017.20

Figure 4.

Figure 4

TMEM65 complementation in patient fibroblast improves mitochondrial function. (a) The representative graph of oxygen consumption rate (OCR) in healthy control fibroblast (black bars) with TMEM65 patient (gray bars) and compared with patient fibroblast transfected TMEM65 expression vector (open bars). The fibroblast from patient showed significantly reduced cellular respiration at baseline, after Oligomycin exposure and after FCCP injection while the oxygen consumption rate was improved in patient fibroblast transfected with TMEM65 expression plasmid. *P<0.01, **P<0.001, ***P<0.0001. (b) Fibroblasts from control and patient were transfected with TMEM65 expression vector and compared for mtDNA copy number and mitochondrial enzyme activity. Enzyme activity and mtDNA copy number were significantly improved in patient after overexpression of TMEM65. *P<0.01, **P<0.001, ***P<0.0001.