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. 2017 Jun 19;18:123. doi: 10.1186/s12931-017-0610-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Provocative methacholine concentrations in mice. Mean FEV_0.1 concentration-response curves to MCh are shown for HDM-asthma and naive controls, with indication of corresponding provocative concentration inducing a 20% (PC20) (a), 30% (PC30) (c), or 40% (PC40) (e) decrease in baseline FEV_0.1. PC20, PC30, and PC40, calculated from the concentration-response data for each individual mouse and group means are shown in b, d, and f, respectively. * p < 0.05, *** p < 0.001 compared to naive control group. n = 7 –8 per group