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. 2017 Jun 8;8:15710. doi: 10.1038/ncomms15710

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Section from the imaged volume through the PV with eight tachyzoites connected to the residual body (rb) outlined by white dashed square. The PV inside the host cell cytoplasm is surrounded by host mitochondria (hm) and located close to the host cell nucleus (hN). Scale bar, 1 μm. (b) High magnification view of the centre of the rosette. The tachyzoite organelles nucleus (N) with nucleolus (n), mitochondrion (m), rhoptry (rh), apicoplast (a) and dense granules (dg) are present. Scale bar, 0.5 μm. (c,d) 3D model of the reconstructed rosette from 33 serial sections of total imaged volume of 18.14 μm × 18.14 μm × 2.31 μm. (c) The individual tachyzoites (cyan) forming the rosette are connected to the residual body (grey) inside the PV (light brown). (d) Same model showing the tachyzoite organelles: nucleus (light blue), mitochondrion (red), rhoptry (orange), apicoplast (green), micronemes (dark blue) and dense granules (dark brown). (e) View of two isolated tachyzoites connected to the residual body with closely apposed mitochondria (red). (f) View from the centre of the residual body showing the connection opening at basal pole of three tachyzoites. The diameter of this opening has been measured (n=111) on several sections of three vacuoles and is estimated at 322±53 nm (mean±s.d.).