Figure 5. Myocardial specific nrg2a overexpression can lead atrial cardiomyocytes to form a multilayered wall.
(a) Illustration of cardiomyocyte specific nrg2a overexpression in zebrafish. (b–i) 2D confocal images (mid-sagittal views) of Tg(myl7:EGFP-Hsa.HRAS) and Tg(myl7:EGFP-Hsa.HRAS);Tg(myl7:nrg2a-p2a-tdTomato) hearts at 50 (b,c), 78 (d,e), 120 (f,g) and 144 hpf (h,i); magnified images of dashed boxes are shown below each time point; arrows point to multilayered walls; At, atrium; V, ventricle; scale bars, 50 μm.