Figure 3.

Statistics of NS3h translocation events on long ssNA. A: Translocation rate measured as a function of applied tension to ssNA. The mean translocation rate and s.e.m. are listed sequentially and respectively, ssDNA: 21.0 ± 2.5 nt/s, 25.5 ± 2.1 nt/s, 26.9 ± 1.2 nt/s, and 27.1 ± 2.9 nt/s; ssRNA: 69.4 ± 6.1 nt/s, 77.2 ± 2.7 nt/s, 78.9 ± 2.8 nt/s, and 78.5 ± 4.3 nt/s. The conversion factors of nm/s to nt/s as a function of tensions were obtained by worm‐like chain modeling. B: Distribution of NS3h translocation rate observed at difference forces. Gaussian functions (light gray and black curve) were fitted to the histograms. Mean and s.d. of ssDNA: 24.3 nt/s and 0.8 nt/s, respectively; mean and s.d. of ssRNA: 74.0 nt/s and 0.7 nt/s, respectively. C: Processivity of NS3h translocation. The histogram shows the fraction of remained molecule after translocating a given distance. The translocation processivity of NS3h was determined by the fitting of exponential decay (light gray and black curve), an inverse of decay constant, 324 ± 9 nt (ssDNA) and 488 ± 13 nt (ssRNA).