Figure 4.

Position‐ramp nanomanipulation of titin. a. Experimental protocol. Titin was first stretched and relaxed with high velocity (pipette movement 200 nm/s), then the pipette was gradually moved toward the optical trap with a rate of 10 nm/s so as to allow titin to shorten slowly. Upper trace, extension as a function of time. Lower trace force as a function of time. The titin molecule refolded completely during the extension‐ramp experiment as evidenced by hysteresis recovery (Supporting Information Fig. S5). b. Enlarged view of the extension‐ramp regime. Raw force data (gray) with superimposed median‐filtered data (smoothing window100‐points, black) are shown. Insets correspond to enlarged views of the boxed data regions.