FlAsH/ReAsH120
|
TetraCystein sequence(TC), 6 aa |
Green,Red |
Incubation |
Covalent, fluorogenic, commericialized |
HaloTag121, 122
|
Bacterial enzyme haloalkane dehalogenase, 30kDa |
Haloalkane conjugated dyes |
Incubation, electroporation |
Covalent, commercialized, enzyme and dye raion 1:1. |
SNAP‐Tag121
|
Human enzyme AGT, 182aa, 19.4kDa |
O6‐benzylguanine conjugated dye123s |
Incubation, electroporation |
Covalent, commercialized |
CLIP‐Tag121
|
Human enzyme, AGT |
O6‐benzylcytosine conjugated dyes |
Incubation, electroporation |
Covalent, modified from SNAP‐tag, commercialize |
eDHFR‐TMP123
|
Bacterial enzyme, eDHFR, 18kDa |
TMP conjugated dyes |
Incubation, electroporation |
Covalent, fluorogenic version available, commercialized |
Metal Chelators124, 125
|
2∼3 kDa |
Nitrilotriacetate conjugated dyes |
Incubation |
Low affinity and low stability, not membrane permeable |