Table 3.
Summary of In Vivo Chemical Dye Labeling Systems
| Name | Protein tagging | Emission | Staining | Comments |
|---|---|---|---|---|
| FlAsH/ReAsH120 | TetraCystein sequence(TC), 6 aa | Green,Red | Incubation | Covalent, fluorogenic, commericialized |
| HaloTag121, 122 | Bacterial enzyme haloalkane dehalogenase, 30kDa | Haloalkane conjugated dyes | Incubation, electroporation | Covalent, commercialized, enzyme and dye raion 1:1. |
| SNAP‐Tag121 | Human enzyme AGT, 182aa, 19.4kDa | O6‐benzylguanine conjugated dye123s | Incubation, electroporation | Covalent, commercialized |
| CLIP‐Tag121 | Human enzyme, AGT | O6‐benzylcytosine conjugated dyes | Incubation, electroporation | Covalent, modified from SNAP‐tag, commercialize |
| eDHFR‐TMP123 | Bacterial enzyme, eDHFR, 18kDa | TMP conjugated dyes | Incubation, electroporation | Covalent, fluorogenic version available, commercialized |
| Metal Chelators124, 125 | 2∼3 kDa | Nitrilotriacetate conjugated dyes | Incubation | Low affinity and low stability, not membrane permeable |