FIG 1.
Reduced expression of Pofut1 in aging and HSACrePofut1FF skeletal muscles. (A) Relative Pofut1 and Pofut2 gene expression in C57BL/6 mouse skeletal muscles at different ages relative to that in young adult (6 weeks old) muscles. (B) Cre immunostaining of HSACrePofut1FF and Pofut1FF muscles at 0, 7, 14, and 21 days and at 17 months. Bar, 200 μm. (C) Western blots of whole-muscle lysates of Gastroc muscle isolated at day 0, 7, 14, or 21, performed by use of two different antipeptide antibodies specific to Pofut1 or an antibody to Cre. (D) Relative Pofut1 and Pofut2 gene expression in HSACrePofut1FF muscles compared to that in HSACre and Pofut1FF muscles. Error bars in panels A and D show standard errors of the means (SEM) (n = 6 to 9 samples per condition). *, P < 0.05; **, P < 0.01; ***, P < 0.001.