FIG 10.

Reduced satellite cell self-renewal in HSACrePofut1^FF muscles. (A) BrdU labeling for 1, 4, 7, 10, or 14 days in HSACrePofut1^FF mice and Pofut1^FF controls was quantified per unit area of labeled TA muscle. (B) BrdU staining in BrdU-labeled (for 14 days) TA muscles was quantified for different ages. (C) Pax7 and BrdU staining and Pax7/BrdU double staining were quantified at different ages. (D) BrdU-labeled cells were quantified 1 day after muscle injury with cardiotoxin. (E) H&E staining of HSACrePofut1^FF and Pofut1^FF muscles 14 days after cardiotoxin injection. The arrow indicates a fat cell, and the asterisk indicates a region rich in extracellular matrix. Bar, 100 μm. Error bars in panels A to D show SEM (n = 6 samples per condition). *, P < 0.05; **, P < 0.01; ***, P < 0.001.