FIG 11.
Division and fusion of cultured HSACrePofut1F/− muscle cells are unchanged. (A) Relative Pofut1 expression in cultured muscle cells isolated from young (3-month-old) and old (23-month-old) C57BL/6 mice, along with markers of muscle differentiation and NotchR activation. Error bars show SEM (n = 9 samples per condition). (B and C) Primary muscle cultures isolated from young (3 months old) Pofut1F/+, Pofut1F/−, and HSACrePofut1F/− mice were compared for growth rate as myoblasts (B) and for myotube fusion after 6 days in fusion medium (C). (D) Images of 6-day fused myotube cultures of Pofut1FF and HSACrePofut1F/− muscles. Bar, 50 μm. *, P < 0.05; ***, P < 0.001.