FIG 12.
Reduced NotchR and Wnt signaling at the neuromuscular junction in HSACrePofut1FF mice. Neuromuscular junctions were identified by α bungarotoxin staining of nicotinic acetylcholine receptors (AChRs) (red). The NotchR intracellular domain (NICD) (A), NotchR1 (A), β catenin (B), Wnt1 (B), and connective tissue growth factor (CTGF) (C) were costained in green. Images of Gastroc muscle are shown. Merged images show coincident staining in yellow, with DAPI stain added to show nuclei. Bars, 200 μm for panels A and B and 100 μm for panel C.