Skip to main content
. 2017 May 2;37(10):e00426-16. doi: 10.1128/MCB.00426-16

FIG 15.

FIG 15

NotchR intracellular domain (NICD) overexpression reduces muscle size. Muscles of 2-month-old Pofut1FF or HSACrePofut1FF mice were injected with AAV-MHCK7-NICD and analyzed at the indicated time points. (A) Western blots of myc-tagged NICD protein in AAV-MHCK7-NICD-injected and mock-injected muscle cell lysates (results at 24 weeks postinjection for Gastroc muscles from 4 different experiments are shown). (B) Relative Pofut1, Pofut2, Hes1, and Hey1 gene expression after NICD overexpression in skeletal muscles. (C) Comparison of muscle weights after NICD overexpression (24 weeks). (D) H&E staining of NICD-overexpressing wild-type (C57BL/6) and HSACrePofut1FF muscles. Images of Quad muscles are shown. Bar, 200 μm. (E) Quantification of distribution of mini-Feret myofiber diameters in NICD-overexpressing wild-type (WT) or HSACrePofut1FF muscles at 24 weeks postinjection. Data for Gastroc muscles are shown. Error bars in panels B and C show SEM (n = 4 samples per condition). *, P < 0.05; **, P < 0.01; ***, P < 0.001.