FIG 5.

Astrocytes invade the La cKO cortex, evidence of astrogliosis. Brain slices (30 μm) from 16-week-old mice were incubated with antibodies directed against GFAP and La. Confocal images were taken with the same laser settings at ×20 magnification. (Bottom) A demarcated region of generally decreased La staining in the cKO cortex, outlined in white (left), corresponds to relatively low levels of GFAP (middle); note that in areas with relatively reduced La staining above the demarcated area, the relatively high levels of GFAP suggest that astrocytes migrated to cells that have lost, or are losing, La protein (see the text).