FIG 2.

FHL2 is associated with the TGF-β1 promoter. (A) Schematic representation of murine TGF-β1 promoter constructs in the luciferase reporter assay. (B) Reporter assay in 293T cells. The activity of the −800/+800 reporter was arbitrarily set at 1. The data are presented as mean induction in luciferase activity plus SD from duplicate samples. The results shown are representative of those from more than three independent assays. (C and D) Association of FHL2 with the murine TGF-β1 promoter. WT and Fhl2^−/− MEFs were analyzed by ChIP-qPCR using chromatin immunoprecipitated by anti-FHL2 antibody or IgG with the primers I (C) and II (D), which are indicated in panel A. (E and F) Association of FHL2 with the human TGF-β1 promoter. HeLa, HepG2, and Huh7 cells were treated with SB431542 to inhibit the effects of endogenous TGF-β, followed by induction for 1 h and 5 h with human TGF-β1 (2 ng/ml), before being subjected to ChIP-qPCR with two independent primers (primer 1 [E] and primer 2 [F]) specific to the human TGF-β1 promoter. *, P < 0.05. The data presented are the means and SD obtained from three independent experiments.