Figure 1.

Experimental protocol for the induction of rhabdomyolysis-induced acute kidney injury in mice. 50% glycerol was administered intramuscularly into the hind limbs of mice at a dose of 10 ml/kg. Saline-injected mice were used as controls. To determine the protective effect of EPO, 500 IU/kg body weight of EPO or phosphate-buffered saline (PBS) was administered intraperitoneally at the indicated time point post-AKI. Forty eight hours after glycerol injection, mice were killed for blood and kidney samples