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. 2017 Apr 6;8(4):e2725. doi: 10.1038/cddis.2017.104

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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EPO inhibited pro-inflammatory response in M1 macrophages. RAW 264.7 cells and bone marrow-derived macrophages (BMDM) were primed with 1 μg/ml LPS and 20 ng/ml IFN-γ for 24 h. EPO were added at indicated concentrations. Secretion levels of (a) NO, (b) TNF-α, (c) IL-1β, and (d) IL-6 were examined by Griess reaction and ELISA. Experiments were performed in triplicate. Data are expressed as mean±S.D. *P<0.05, **P<0.01