Figure 2.

SRT1720 pretreatment promotes the survival of aged hMSCs under stress in vitro and in vivo. Protective effect of various concentration (0, 0.05, 0.1, 0.2, 0.5, 1, 2 μM) of SRT1720 was evaluated under the situations of serum deprivation (a), serum deprivation and hypoxia (b), serum deprivation and H₂O₂ (c) by CCK-8 analysis. The survivals of aged hMSCs pretreated with 0.5 μM SRT1720 for different time (0, 3, 6, 12, 24, 36 h) are detected under serum deprivation combined H₂O₂ situation (d). SRY gene expression of hMSCs in the whole heart was analyzed at day 1 (e) and day 3 (f) after cell therapy. The control condition (Ctrl) was normal culture medium (DMEM with 10% FBS). The volume fraction of DMSO was the same in every groups. Data are expressed by mean±S.D. (three independent experiments, N=3). *P<0.05 versus DMSO group; **P<0.01 versus DMSO group; ^#P<0.05