Figure 6.

SRT1720 pretreatment protects aged hMSCs from apoptosis through upregulating FAIM. The expression of genes associated with cell survival and proliferation were confirmed by real time PCR (a). The expression of FAIM after SRT1720 pretreatment was tested by western blot (b and c). The reversal of the changed induced by SRT1720 was also evaluated when FAIM was silenced with western blot (d–f). Representative images of Hoechst 33258 staining (g) and quantitative analysis (h) are shown for apoptosis examination. Ctrl refers to normal culture medium. The other stress groups are treated with serum deprivation plus H₂O₂ medium. The concentration of DMSO (solvent of SRT1720 and EX-527) was the same in every groups. Greek letters refer to the fold change in H₂O₂+SRT1720 group versus H₂O₂+DMSO group, α=14.89±0.24; β=8.07±0.19; γ=12.34±0.31; δ=12.64±0.21; ɛ=8.05±0.25; η=2.31±0.22; θ=11.64±0.27. Data are expressed by mean±S.D. (three independent experiments, N=3). *P<0.05; **P<0.01