Figure 1.

Altered p45 NF-E2 expression and GCM1 acetylation in human IUGR placentae. (a and b) Increased syncytial knot formation/mm² in IUGR placentae compared with controls (H&E staining; (a) representative images; (b) bar graph summarizing results). (c) p45 NF-E2 in downregulated in placentae obtained from women with pregnancies complicated with IUGR compared with controls (representative immunofluorescence images; red: p45 NF-E2; blue: DAPI nuclear staining). (d and e) Reduced expression of p45 NF-E2 and enhanced lysine-acetylation in placenta tissue lysates obtained from women with pregnancies complicated with IUGR compared with controls. GCM1 expression did not differ significantly between the two cohorts (immunoblot analysis; (d), representative immunoblots; (e), dot-plot summarizing results). (f and g) Gcm1 acetylation is increased in placenta tissue lysates obtained from women with pregnancies complicated with IUGR compared with controls; immunoprecipitation using anti-GCM1 antibody followed by immunoblotting ((f) representative immunoblots; (g) box-plot summarizing results). Size bars represent 100 μm (a) and 20 μm (c); mean±S.E.M. (b,e and g); *P<0.05, **P<0.01 (t-test in b and g; Mann–whitney U-test in e)