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. 2017 Apr 6;8(4):e2730. doi: 10.1038/cddis.2017.127

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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p45 NF-E2 regulates syncytiotrophoblast differentiation of human trophoblast cells in vitro.(a and b) Enhanced syncytia formation after 8-Br-cAMP treatment in BeWo cells; immunofluorescence staining ((a) representative images, red: E-cadherin, blue: DAPI nuclear staining; (b) bar graph summarizing results). (c and d) Downregulation of p45 NF-E2 expression after 8-Br-cAMP treatment in BeWo cells and primary human villous trophoblast cells (pHVT) accompanied by upregulation of differentiation markers GCM1, Syn-1 and hCG-β expression, indicating increased syncytiotrophoblast formation compared with control BeWo cells (immunoblotting analysis; (c) representative immunoblots; (d) bar graph summarizing results). (e and f) 8-Br-cAMP induces acetylation of GCM1. Immunoprecipitation using anti-GCM1 antibody followed by immunoblotting for acetylated lysine ((e) representative immunoblots; (f) bar graph summarizing results) showing enhanced GCM1 acetylation after treatment of BeWo cells and primary human villous trophoblast cells (pHVT) with 8-Br-cAMP. Size bars represent 50 μm (a); mean±S.E.M. (b,d and f); results of at least five independent repeat experiments shown (b,d and f); *P<0.05 (t-test in b,d and f)