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. 2017 Apr 6;8(4):e2730. doi: 10.1038/cddis.2017.127

Figure 5.

Figure 5

Overexpression of p45 NF-E2 restricts syncytiotrophoblast differentiation in human trophoblast cells. (a and b) p45 NF-E2 overexpression (pCMV-NF-E2) prevents 8-Br-cAMP induced GCM1 and Syn-1 expression, indicating reduced syncytiotrophoblast formation (immunoblot analysis; (e) representative immunoblots; (f) bar graph summarizing results). (c and d) Overexpression of p45 NF-E2 prevents 8-Br-cAMP-induced acetylation of GCM1. Immunoprecipitation using anti-GCM1 antibody followed by immunoblotting ((g) representative immunoblots; (h) bar graph summarizing results) showing reduced expression of acetylated GCM1 after overexpression of p45 NF-E2. Treatment of BeWo cells overexpressing p45 NF-E2 with 8-Br-cAMP did not increase GCM1 acetylation. Mean±S.E.M. (b and d); results of at least five independent repeat experiments shown (b and d); *P<0.05 (ANOVA in b and d)