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Extracellular CST1 is required in the prevention of cellular senescence. MDA-MB-231 cells transduced with a control shRNA (mock) or an shRNA for CST1 were cultured with an MDA-MB-231 culture supernatant (a) or CST1-overexpressing MCF-7 culture supernatant (b). (c) The effect of recombinant CST1-immunoglobulin (rCys-SN) on CST1 knockdown-mediated cellular senescence. Scale bars, 50 μm. *P<0.05; **P<0.01; ***P<0.001 by Student's t-test
