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. 2017 Apr 6;8(4):e2729. doi: 10.1038/cddis.2017.153

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Cellular senescence is induced by GS activation resulted in glycogen accumulation in CST1 knockdown cells. The following assays were performed in CST1 knockdown MDA-MB-231 cells transfected with short-interfering RNAs (siRNAs) for green fluorescent protein (GFP) or GS. (a and d) The expression level of the indicated protein was analyzed by western blotting. (b) PAS staining analysis was performed to assess glycogen accumulation. (c) Quantification of cellular senescence was determined by the SA-β-gal assay. (e) Expression of SASP genes, IL-6 and CCL20, was analyzed by real-time polymerase chain reaction. The results were normalized to the level of β-actin. Scale bars, 50 μm. *P<0.05; **, P<0.01, *** P<0.001 by Student's t-test