Figure 3.

The effect of TBK1 overexpression on p16 expression and cell senescence in vitro. (a) Western blot showing the expression of TBK1, p16 and cyclin A after H₂O₂ (100 μM) stimulation in HEK293. (b-d) Statistical analysis of the data shown in (a). The data are expressed as the means±s.e.m. from three independent experiments, *P<0.05 versus control. (e) Western blot showing the expression of TBK1, p16 and cyclin A after LPS stimulation in HEK293. (f-h) Statistical analysis of the data shown in (e).The data are expressed as the means±s.e.m. from three independent experiments, *P<0.05 versus control. (i) Western blot showing the effect of TBK1 overexpression on TBK1, p16 and cyclin A expression in HEK293. (j-l) Statistical analysis of the data shown in (i).The data are expressed as the means±s.e.m. from three independent experiments, *P<0.05 versus control. (m) Representative β-gal staining images showing the effect of TBK1 overexpression on HEK293 cell senescence