Figure 5.

miR-10a negatively regulates the expression levels of MMP14 and ACTG1. (a and b) The mRNA and protein levels of MMP14 and ACTG1 in SW480 and SW620 cells were detected. The mRNA level was normalized to β-actin and the protein level was normalized to GAPDH. The data represented three measurements. Results are expressed as mean±S.D. *P<0.05. (c) The mRNA levels of MMP14 and ACTG1 in SW480 cells transfected with ASO-miR-10a or ASO-Ctrl and pri-miR-10a or Ctrl were detected. Results are expressed as mean±S.D. *P<0.05. (d) The protein levels of MMP14 and ACTG1 in SW480 cells transfected with ASO-miR-10a or ASO-Ctrl and SW620 cells transfected with pri-miR-10a or Ctrl were examined. Results are expressed as mean±S.D. *P<0.05. (e) Pearson's correlation analysis of the negative correlation between the expression of miR-10a and MMP14/ACTG1 (*P<0.05). (f) Representative IHC images of MMP14 and ACTG1 in primary CRC tissues and paired lymph node metastases are shown