Figure 7.

MMP14 and ACTG1 promotes the anoikis resistance of SW620 cells by upregulating Bcl-2. (a) RT-qPCR assay of miR-10a levels in SW620 cells transfected with pri-miR-10a and ASO-miR-10a compared with the control groups (*P<0.05, **P<0.01). (b) The protein levels of MMP14 and ACTG1 induced by pri-miR-10a and ASO-miR-10a in SW620 cells. Represent images and histogram were showed. The data represented three measurements (*P<0.05, **P<0.01). (c) FACS results of the apoptosis of SW620 cells in each group, as indicted. Quantitative results of three independent experiments are shown (*P<0.05, **P<0.01, NS, not significant). (d) Western blot results for β-integrin, Bcl-2, MMP14 and ACTG1 in the indicated SW620 cells. The data represented three measurements. Results are expressed as mean±S.D. (*P<0.05 and **P<0.01 versus si-NC group, ^#P<0.05 versus ASO-Ctrl group, NS, not significant versus si-NC group). (e) Schematic describing the roles of MMP14 and ACTG1 as regulated by miR-10a in CRC metastasis