Figure 3.

Xenogeneic human cells robustly induce leukemia in secondary transplantation assays. Fresh xenogeneic cells derived from murine BM purified by MACS recognizing leukemic antigens were retransplanted at equal numbers as in the corresponding primary transplantation assay. Note that robust engraftment occurred from all retransplanted cases, including the long-latency engrafting AML. Of note, engraftment occurred faster (left) and mice survived for a shorter time (right) after secondary transplantation (blue line) than after the primary transplantations (red line). This is also summarized in the table below. A log-rank (Mantel-Cox) test was applied to calculate statistical significance.