Figure 5.
The diversity of cold, menthol, and AITC responses from HEK293 cells stably expressing either human TRPA1 or TRPM8 channels. The experimental protocol is identical to that described in the Figure 1 legend. (A) Representative traces from human TRPA1-expressing HEK cells. A subset responded to cold, menthol and AITC (47.2% ± 2.2%). Another subset responded to menthol and AITC (34.0% ± 2.1%). An additional subset only responded to AITC (14.8% ± 1.6%). See summary in Table 1. (B) Representative traces from human TRPM8-expressing HEK cells. A subset of these neurons responded to 18 °C and 4 °C bath applications, and menthol (89.8% ± 1.0%). Another subset only responded to 4 °C and menthol (9.2% ± 1.0%). See summary in Table 1. (C) Representative trace from untransfected HEK cells.