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. Author manuscript; available in PMC: 2018 Jun 14.
Published in final edited form as: Neuroscience. 2017 Apr 10;353:76–86. doi: 10.1016/j.neuroscience.2017.04.001

Figure 6.

Figure 6

The frequency of responses to cold increased in TRPA1-expressing (AITC responsive) DRG neurons from TRPM8−/− mice when potassium channels that contain KV1.2 subunits were blocked by κM-conotoxin RIIIJ (RIIIJ). Mouse DRG neurons that responded to AITC (n=796) were challenged with a 4 °C bath solution for 2-minutes prior to six-minute incubation with 1 μM RIIIJ and then again after incubation with RIIIJ in the continued presence of RIIIJ (black bar). The 2-minute cold ramp was then repeated again in the presence of RIIIJ and the TRPA1 blocker, HC030031 (white bar). (A) The bar graph shows the percentage of AITC-responsive neurons that also responded to the 4 °C bath solution, 4 °C in the presence of RIIIJ, and 4 °C in the presence of RIIIJ and HC030031. (B) Representative calcium imaging traces and experimental protocol used to obtain the data in A. In these experiments, the bath solution was cooled to a temperature of 4 °C for 2-minutes, according to the temperature ramp shown on the time-scale of the X-axis. The temperature was monitored with a thermocoupler in the bath near the imaging field of view. Dashed lines indicate times when temperature data was not monitored in the bath because the bath solution was replaced by the room-temperature solution.