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. Author manuscript; available in PMC: 2018 Jun 14.
Published in final edited form as: Neuroscience. 2017 Apr 10;353:76–86. doi: 10.1016/j.neuroscience.2017.04.001

Figure 7.

Figure 7

Representative traces of cold-sensitive menthol-and AITC-unresponsive (CS M−A−) neurons. The experimental protocol shown below traces is similar to the protocol in Figure 6, where the cold ramp was applied for 2-minutes with and without κM-RIIIJ, a selective blocker of KV1.2 containing channels (black bar), and HC-030031, a TRPA1 blocker (white bar). About 7% ± 2% of K+ responsive neurons (n=3364) that were unresponsive to menthol or AITC responded to a 2-minute cold ramp to 4 °C as illustrated by the top trace. In the presence of κM-RIIIJ, the frequency of CS M−A− neurons increased to approximately 12% ± 1% of DRG neurons, as shown in the middle trace, while the majority of menthol-and AITC-unresponsive neurons remained unaffected as shown in the bottom trace. The temperature was monitored with a thermocoupler in the bath near the imaging field of view. Dashed lines indicate times when temperature data was not monitored in the bath because the bath solution was rep laced by the room-temperature solution.