Table 2.
Response phenotypes of AITC+ (TRPA1-expressing) and Menthol+, AITC−(TRPM8-expressing) mouse DRG neurons as percentages of the total neuronal cell population.
| WT Mice | TRPM8 −/− Mice | |||
|---|---|---|---|---|
| % K+ (n=1465) | SEM | % K+ (n=1535) | SEM | |
| 4°+ M+ A+ | 2.9% | 0.4% | 2.1% | 0.4% |
| 4°− M+ A+ | 7.2% | 0.7% | 9.8% | 0.8% |
| 4°− M− A+ | 26.5% | 1.2% | 18.9% | 1.0% |
| 4°+ M− A+ | 0.3% | 0.2% | 0.4% | 0.2% |
| Other Neurons | 63.1% | 1.3% | 68.9% | 1.2% |
| Total Neurons | 100.0% | 100.0% | ||
| WT Mice | TRPA1 −/− Mice | |||
|---|---|---|---|---|
| % K+ (n=1465) | SEM | % K+ (n=1107) | SEM | |
| 18°+ 4°+ M+ A− | 5.3% | 0.6% | 3.2% | 0.5% |
| 18°− 4°+ M+ A− | 2.4% | 0.4% | 2.7% | 0.5% |
| 18°− 4°− M+ A− | 0.1% | 0.1% | 0.8% | 0.3% |
| Other Neurons | 92.2% | 0.7% | 93.3% | 0.8% |
| Total Neurons | 100.0% | 100.0% | ||
Abbreviations are the following: 18°, 18° C for 15 seconds; 4°, 4° C for 30-seconds; M, Menthol; A, AITC; +, responsive; −, not responsive; n, number of cells; SEM, standard error of the mean.
The percentages in the table were calculated from the following:
WT Mice: 6 experimental trials from 3 cell cultures (mice).
TRPM8 −/− mice: 7 experimental trials from 4 cell cultures (mice).
TRPA1 −/− mice: 8 experimental trials from 3 cell cultures (mice).