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. 2017 Apr 14;6(2):167–174. doi: 10.1080/21623945.2017.1319535

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© 2017 Taylor & Francis

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Figure 1. — A typical respiration protocol to determine the oxygen consumption rate (OCR) of mitochondria (isolated organelles or permeabilized tissue) in the leak and coupled state (Panel A). The respiratory states being assayed following the sequential added of substrates, ADP and the ATP synthase inhibitor oligomycin are shown at the top of the oxygraph trace. In Panel B, a similar oxygraph trace is shown, with the exception that this protocol is designed to assay respiration in the leak, coupled and uncoupled states following the sequential added of substrates, ADP and an ionophore, such as FCCP.