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S. sonnei growth under various osmotic conditions. Growth curves of wild type (triangle), proV mutant (gray square), MirproV (RNAi proV strain) (gray trigonal) and ΔproV /pProV strains (circle) in M9 medium supplemented with 0.3 M NaCl (A), 0.3 M KCl (B), 0.2 M NaCl (C, E) and 0.2 M KCl (D, F) in the presence (open symbols) or absence (closed symbols) of 500 µM betaine (C, D, E, F). The results are means of 3 successive wells ± standard deviation (n = 3).
