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Deletion of proV doesn't disturb the function of TTSS. S. sonnei wild type, the complemented strain ΔproV/PproV, ΔmxiD, ΔproV and MirproV strains were grown to mid-log phase, and TTSS secretion was induced with Congo red. Total proteins from cell lysates (A) and culture supernatants (B) were separated on SDS-PAGE and IpaB and IpaC were detected with anti-IpB and anti-IpaC antibodies.
