Figure 3.

Toe-printing assays verified the frameshifting. The toe-printing assays of the cDNAs that were reverse transcribed with Cy5-labeled primer. Lane 1: markers of 32 and 55 nt in lengths; Lanes 2, 3, and 4: toe-printing of SLP, GA₇G, and AGU sequences, respectively. The right panel was a close-up view that was obtained by averaging four repeated scans. In Lane 4, the distinct bands were consistent with the decoding of K, S, E, and M, respectively. The bands near 106-nt at the top of the plots were the cDNAs reverse transcribed to the 5′-of the mRNAs.