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. 2017 Apr 24;12(6):1629–1635. doi: 10.1021/acschembio.7b00028

Figure 4.

Figure 4

Products of the second- and third-step translocations. (a) FIRMS profile showing the formation of Post2(−1) and Post2(−2), indicated by the 13-bp dissociation at 42 pN and the 14-bp dissociation at 57 pN, respectively. (b) FIRMS profiles showing the formation of Post3(0) via Post2 only in the presence of EF-G·GTP, indicated by the 12-bp duplex. YWG: mix of Y-tRNAtyr, Tu·GTP, and EF-G·GTP. Y0G: mix of Y-tRNAtyr and Tu·GTP only, without EF-G·GTP.