Figure 2.

Electron micrographs of mouse Bruch's membrane of 9-months-old Efemp1^+/+ (+/+), Efemp1^ki/ki (ki/ki), and Efemp1^−/− (−/−) mice treated with C-ABC and stained by CB. Note that the filaments representing C/DSPGs in the fibrous layers of Bruch's membrane were eliminated by C-ABC treatment, but that filaments (arrows) representing HSPGs in the basement membranes of the RPE and the endothelium of choriocapillaris were preserved. BI, basal infolding; BM, basement membrane; EN, endothelium. Scale bar: 500 nm.