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Electron micrographs of mouse Bruch's membrane of 9-month-old Efemp1+/+ (+/+), Efemp1ki/ki (ki/ki), and Efemp1−/− (−/−) mice treated with C-ABC and nitrous acid coupled by CB staining. Note that all the filaments in Bruch's membrane were eliminated. Black dots distinct from the filaments representing proteoglycans in +/+ and ki/ki samples were likely background staining. BI, basal infolding; BM, basement membrane; EN, endothelium. Scale bar: 500 nm.
