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. 2017 Feb 28;17:56. doi: 10.1186/s12870-017-1003-9

Table 2.

Effect of EBR seed-soaking on the contents of stress markers and glutathione along with activities of antioxidative enzymes in Brassica juncea seedlings grown in IMI supplemented substratum (Mean ± SD, Two-way ANOVA, Tukey’s HSD, multiple linear regression)

Treatments H2O2 content (μmol g−1 FW) O. 2 − content (μmol g−1 FW) SOD activity (Units mg−1 protein) CAT activity (μmol min−1 mg−1 protein) POD activity (μmol min−1 mg−1 protein) GR activity (μmol min−1 mg−1 protein) GST activity (μmol min−1 mg−1 protein) GSH content (mg g−1 FW)
IMI (mg L−1) EBR (nM)
0 0 12.70 ± 1.27 0.97 ± 0.15 29.27 ± 3.29 5.68 ± 0.74 35.10 ± 2.88 5.91 ± 0.24 26.48 ± 1.06 0.51 ± 0.02
0 100 11.48 ± 1.87 0.88 ± 0.12 38.85 ± 4.95 7.57 ± 0.83 52.94 ± 4.60 8.51 ± 0.59 34.20 ± 2.33 0.75 ± 0.05
150 0 15.22 ± 1.53 1.64 ± 0.29 36.25 ± 5.03 6.71 ± 0.98 44.14 ± 5.19 7.83 ± 0.60 28.99 ± 1.52 0.56 ± 0.03
150 100 10.11 ± 0.35 1.02 ± 0.20 53.98 ± 6.24 9.93 ± 1.14 71.46 ± 4.85 10.81 ± 0.66 37.47 ± 2.51 0.69 ± 0.03
200 0 17.41 ± 3.17 2.56 ± 0.52 32.85 ± 3.52 5.19 ± 0.85 68.23 ± 8.52 7.91 ± 0.41 31.25 ± 1.25 0.50 ± 0.04
200 100 10.94 ± 1.61 1.64 ± 0.17 50.72 ± 6.23 8.94 ± 0.50 94.40 ± 10.41 9.66 ± 1.20 38.67 ± 2.13 0.72 ± 0.07
250 0 21.70 ± 0.47 3.14 ± 0.60 27.98 ± 5.22 3.50 ± 0.74 43.93 ± 3.71 5.88 ± 0.64 25.71 ± 1.01 0.39 ± 0.06
250 100 14.81 ± 0.86 1.75 ± 0.29 42.67 ± 3.43 6.47 ± 0.78 74.28 ± 8.45 7.11 ± 1.03 32.89 ± 0.98 0.62 ± 0.04
FIMI
FEBR
FIMI×EBR
HSD (p < 0.05)		 17.53***
54.84***
3.77*
4.59		 25.53***
30.32***
3.98*
0.95		 6.99**
56.66***
0.95
13.77		 16.14***
74.60***
1.32
2.31		 33.01***
89.59***
0.99
18.60		 19.73***
51.55***
1.77
2.06		 14.00***
122.45***
0.16
4.81		 10.79***
128.84***
2.03
0.08
Multiple linear regression equation β-regression coefficients Multiple correlation coefficient Significant at p <
βIMI βEBR
H2O2 content = 13.6710 + 0.0205 X1–0.0490 X 2 0.4994 –0.6379 0.8102 0.001
O. 2 − content = 1.1777 + 0.0060 X1–0.0080 X 2 0.7037 –0.4741 0.8485 0.001
SOD activity = 29.388 + 0.0146 X1 + 0.1497 X 2 0.1403 0.7662 0.7790 0.001
CAT activity = 5.8784–0.0041 X1 + 0.0296 X 2 –0.1852 0.7222 0.7465 0.001
POD activity = 33.3930 + 0.0963 X1 + 0.2542 X 2 0.4660 0.6569 0.8054 0.001
GR activity = 6.8544 + 0.0002 X1 + 0.0214 X 2 0.0110 0.6247 0.6249 0.01
GST activity = 27.562 + 0.0036 X1 + 0.0770 X 2 0.0725 0.8226 0.8258 0.001
GSH content = 0.5507–0.0004 X1 + 0.0021 X 2 –0.3077 0.8384 0.8931 0.001

X1 = IMI (mg L−1), X 2 = EBR (nM), *, ** and *** = significant at p < 0.05, p < 0.01 and p < 0.001 respectively